1499 - 1556 (57 years)
| Eleonore De Toledo | |
| B: | 1521 |
| D: | 18 Dec 1562 |
| Pisa, Spain | |
| Cosimo I Medici | |
| B: | 12 Jun 1519 |
| D: | 21 Apr 1574 |
| Castello, Spain | |
| Maria De Osorio | |
| B: | 1499 |
| D: | 1556 |
| Pedro De Toledo | |
| B: | 1493 |
| D: | 1559 |
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